Sample information |
|
Picture |
|
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Location | |
Collection date | 08/22/2022 |
Captive / Cultivated? | Wild-caught |
Group | Kantonsschule Zuercher Unterland |
Observations | In a bush |
Putative identification | Arthropoda Chelicerata Arachnida Araneae Salticidae Ballus Ballus chalybeius |
Methods |
|
Extraction kit | Instagene Matrix |
DNA extraction location | Rear half |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | MiniOne |
Buffer | TBE |
DNA stain | Fast Blast |
Gel images |
|
Protocol notes | DNA stain: Midori 1:20000 All went well
|
Results |
|
Wolbachia presence | No |
Confidence level | Medium |
Explanation of confidence level | We did it in school so we are not sure about how exact everything was done. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
|
Summary | The Ballus chalybeius was found to be negative for Wolbachia. |