Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/22/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Kantonsschule Zuercher Unterland |
| Observations | In a bush |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae Salticidae Ballus Ballus chalybeius |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Rear half |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | DNA stain: Midori 1:20000 All went well
|
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | We did it in school so we are not sure about how exact everything was done. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Ballus chalybeius was found to be negative for Wolbachia. |
Water Boatman