Nathan Fruit Fly

Sample information

Picture
Location No map location data available.
Collection date
Captive / Cultivated? Captive / Cultivated
Group St. Albans School
Observations
Putative identification Arthropoda Hexapoda Insecta Diptera Drosophilidae Drosophila

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

was given by the Weinstein lab as a negative control, also all controls worked with a great ladder

Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
NNNNNNNNTGCTGGAAGTTGGAACATCTTTAAGAATTTTAATTCGAGCTGAATTAGGACATCCTGGAGCATTAATTGGAGATGATCAAATTTATAATGTAATTGTAACTGCACATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGTGGATTTGGAAATTGATTAGTGCCTTTAATATTAGGTGCTCCTGATATAGCATTCCCACGAATAAATAATATAAGATTTTGACTTCTACCTCCTGCTCTTTCTTTACTATTAGTAAGTAGAATAGTTGAAAATGGAGCTGGGACAGGATGAACTGTTTATCCACCTCTATCCGCTGGAATTGCTCATGGTGGAGCTTCAGTTGATTTAGCTATTTTTTCTCTACATTTAGCAGGAATTTCTTCAATTTTAGGAGCTGTAAATTTTATTACAACTGTAATTAATATACGATCAACAGGAATTTCATTAGATCGTATACCTTTATTTGTTTGATCAGTAGTTATTACTGCTTTATTATTATTATTATCACTTCCAGTACTAGCAGGAGCTATTACTATATTATTAACAGATCGAAATTTAAATACATCATTTTTTGACCAGCGGGAGGAGGAGATCCTATTTTATACCACATTTTTTGTTTNNNNNNNNANANNNANTNNNAAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Drosophila was found to be negative for Wolbachia.
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