Sample information |
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Picture |
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Location | No map location data available. |
Collection date | |
Captive / Cultivated? | Captive / Cultivated |
Group | St. Albans School |
Observations | |
Putative identification | Arthropoda Hexapoda Insecta Diptera Drosophilidae Drosophila |
Methods |
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Extraction kit | DNeasy (Qiagen) |
DNA extraction location | Whole arthropod |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | MiniPCR |
Buffer | TBE |
DNA stain | GelGreen |
Gel images |
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Protocol notes | |
Results |
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Wolbachia presence | No |
Confidence level | High |
Explanation of confidence level | was given by the Weinstein lab as a negative control, also all controls worked with a great ladder |
Wolbachia 16S sequence | |
Arthropod COI sequence | Download FASTA
Download AB1
NNNNNNNNTGCTGGAAGTTGGAACATCTTTAAGAATTTTAATTCGAGCTGAATTAGGACATCCTGGAGCATTAATTGGAGATGATCAAATTTATAATGTAATTGTAACTGCACATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGTGGATTTGGAAATTGATTAGTGCCTTTAATATTAGGTGCTCCTGATATAGCATTCCCACGAATAAATAATATAAGATTTTGACTTCTACCTCCTGCTCTTTCTTTACTATTAGTAAGTAGAATAGTTGAAAATGGAGCTGGGACAGGATGAACTGTTTATCCACCTCTATCCGCTGGAATTGCTCATGGTGGAGCTTCAGTTGATTTAGCTATTTTTTCTCTACATTTAGCAGGAATTTCTTCAATTTTAGGAGCTGTAAATTTTATTACAACTGTAATTAATATACGATCAACAGGAATTTCATTAGATCGTATACCTTTATTTGTTTGATCAGTAGTTATTACTGCTTTATTATTATTATTATCACTTCCAGTACTAGCAGGAGCTATTACTATATTATTAACAGATCGAAATTTAAATACATCATTTTTTGACCAGCGGGAGGAGGAGATCCTATTTTATACCACATTTTTTGTTTNNNNNNNNANANNNANTNNNAAA
BLAST at The Wolbachia Project BLAST at NCBI
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Summary | The Drosophila was found to be negative for Wolbachia. |