Lophocampa maculate- Spotted tussock moth(caterpillar)

March 11, 2023

Sample information
Picture
Photos by: Chase Hartzell, Heather Ahlstrom
Location
Collection date 09/06/2022
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations

Lophocampa maculate- Spotted tussock moth(caterpillar) On trees with bright green long leaves. Many grouped next to the river.

Description: long hairs black and yellow-orange striped.

Body Length: 2.8 cm. Found by Carly Hartzell
Putative identification Arthropoda Hexapoda Insecta Lepidoptera Erebidae Lophocampa Lophocampa maculata

Methods
Extraction kit Edwards Buffer
DNA extraction location Rear half
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Wolbachia Project PCR Primers for both Wolbachia and CO1 Arthropod.
Wolbachia 16S sequence
Arthropod COI sequence
>C01_8c_CspecF GGATTTGAGCAGGAATAGTAGGAACTTCATTAAGATTATTAATTCGAGCTGAATTAGGAAATCCTNGNTCTTTAATTGGAGATGATCAAATTTATAATACTATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCAATTATAATTGGAGGATTTGGTAATTGATTAATTCCTTTAATGTTAGGAGCTCCTGATATAGCTTTTCCTCGAATAAATAATATAAGTTTTTGACTTCTTCCCCCATCTTTAACTTTATTAATTTCAAGAAGAATTGTAGAAAATGGAGCAGGAACAGGATGAACAGTTTACCCCCCACTTTCATCTAATATTGCTCATGGAGGTAGATCAGTAGATCTAGCTATTTTTTCTTTACATTTAGCAGGAATTTCTTCTATTCTAGGAGCAATTAATTTTATTACAACAATTATTAATATACGATTAAATAATTTATCTTTTGATCAAATACCTTTATTTATTTGAGCCGTAGGAATTACAGCATTTTTATTATTACTTTCTTTACCAGTATTAGCAGGAGCAATTACAATATTATTAACTGATCGAAATTTAAATACATCATTTTTTGATCCAGCTGGAGGAGGAGATCCTATTCTTTATCAACATTTATTTTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Lophocampa maculata was found to be negative for Wolbachia.
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