Sample information
Picture	Photos by: Emily P.
Location
Collection date	10/05/2022
Captive / Cultivated?	Wild-caught
Group	Elizabethtown Area High School
Observations	male mosquito
Putative identification	Arthropoda Hexapoda Insecta Diptera Culicidae Aedes Aedes albopictus
Methods
Extraction kit	Wizard SV (Promega)
DNA extraction location	Whole arthropod
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	Other
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Low
Explanation of confidence level	Bands were faint and difficult to interpret, but controls still worked.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Aedes albopictus was found to be negative for Wolbachia.