GRE-ACF-SP23-18 Diptera

April 19, 2023

Sample information
Picture
Photos by: JaNiya R.
Location No map location data available.
Collection date 01/25/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
Date: 1/25/233
Time: 1:22PM
Location: Greenhouses to the left of the front parking lot at the science center.
Environment: Tall grassy areas with overcast of rain
Method: Sweeping Net and Jar
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

A DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

All of my control results came out as expected.
Wolbachia 16S sequence
CGAGAACGTATTCACCGTGGCATGCTGATCCACGATTACTAGCGATTcccCttcatgcacTCGAG TTGCAGAGTACAATCCGAACTGAgacctttTTTAGGGATTAGCTTAGGCTTGCGCACCTTGCAGC CCATTGTAGACACCATTGTAGCACGTGTGTAGCCCaCTTCATAAAGGCCATGATGACTTGACATC ATCCCCATCTTCCTCCAGCTTATCACTGGCAGTTTCCTTAAAGTGCTCAGCATTACCTGATGGCA ACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGAC AGCCATGCAACACCTGTGTAAAATCCGGCCGAACCGACCCTATCCCTTCGAATAGGTATG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
TATTTGGAGCATGAGCAGgaataGTAGGAACATCTCTAAGAATTTTAATTCGAGCTGAATtacgt ttcCCAGGAGCTTTAATTGGAGATGATCAAATTTATAAccttattGTTACTGCACATGCTTTTGT AATAATTTTTTTTATAGTAATACCAATTATAATTGGAGGATTTGGAAATTGACTAGTCCCATTAA TATTAGGAGCACCTGATATAGCATTTCCTCGAATAAATAATATAAGTTTTTGATTACTTCCACCA TCATTAACACTTTTAATGGCTAGAAGTATAGTGGAAAATGGAGCCGGAACAGGATGAACTGTATA TCCACCTTTATCTTCAATTATTGCACATGGGGGAGCTTCAGTAGATTTAGCTATTTTTTCTCTTC ATTTAGCTGGGGTATCATCAATTTTAGGAGCAGTAAATTTTATTACTACAGTAATTAATATACGA TCTACAGGAATTACATTCGATCGAATACCATTATTTGTATGATCAGTAGTAATTACTGCTTTATT ATTACTATTATCACTACCAGTATTAGCAGGAGCCATTACTATATTATTAACTGATCGAAATTTAA ATACTTCATTCTTTGATCCTGCAGGAGGAGGGGATCCAATTTTATATCAACATTTATTTTGATTT TTTGGTCA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be postive for Wolbachia.
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