Diamesa nivoriunda

Sample information

Photos by: Sofi M.
Collection date 03/31/2023
Captive / Cultivated? Wild-caught
Group Woodbridge High School

Black midge. About 1 cm in length.

Putative identification Arthropoda Hexapoda


Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes


Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls worked, which was how I knew this arthropod was Wolbachia negative.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hexapoda was found to be negative for Wolbachia.
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