Sample information
Picture	Photos by: Sydney B.
Location
Collection date	04/04/2023
Captive / Cultivated?	Wild-caught
Group	Woodbridge High School
Observations	Fast, small, lived amongst leafy areas.
Putative identification	Arthropoda Hexapoda
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	Band was below 438bp
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Hexapoda was found to be negative for Wolbachia.