Wolbachia Capstone for Carpenter Ant

April 27, 2023

Sample information
Picture
Photos by: Sydney B.
Location
Collection date 04/04/2023
Captive / Cultivated? Wild-caught
Group Woodbridge High School
Observations

Fast, small, lived amongst leafy areas.
Putative identification Arthropoda Hexapoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Band was below 438bp
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hexapoda was found to be negative for Wolbachia.
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