Stubenfliege (Palearctic sawfly, 003)

October 20, 2020

Sample information
Picture
Location
Collection date 09/28/2020
Captive / Cultivated? Wild-caught
Group Unterstrass
Observations
  • Collected from forest, air
  • Autumn
  • 15°C
  • Elevation – 460
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Tenthredinidae Athalia Athalia cordata

Methods
Extraction kit Wizard SV (Promega)
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
CAGGATACTTGGACTATCACTAAGCATGATTATCCGAAGAGAATTAAGATCAGCAAATTCACTTATTAATAATGATCAAGTTTATAACGTAATTGTTACATCTCACGCTTTCTTAATAATTTTCTTCATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGATTAATTCCATTAATATTAGGAGCACCCGATATAGCATTCCCACGAATAAATAATATAAGATTCTGATTTTTACCTCCATCCCTAATTCTACTTCTTTCAAGAAGAATAGTAAACACTGGATCTGGAACAGGGTGAACAGTTTATCCACCATTATCCTCAAATACCTCCCATGCTGGAGCATCTGTTGATTTAACAATTTTTTCATTACATTTAGCAGGTATTTCTTCCATTTTAGGAGCTATTAACTTTATTTCTACTATTTTAAATATACGAATAAATGGTATAACAAACGAACGAATAAGTTTATTCACATGATCAGTATTATTAACAGCTATTTTATTATTATTATCATTACCAGTGCTAGCAGGAGCAATTACAATATTATTAACAGATCGAAATTTAAATACATCATTTTTTGACCCTTCTGGAGGTGGAGACCCCATTCTATATCAACATCTATTTTGATTTTTTGGTCAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Athalia cordata was found to be negative for Wolbachia.
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