brauner Grashüpfer (Grasshopper, 008)

October 20, 2020

Sample information
Picture
Location
Collection date 09/28/2020
Captive / Cultivated? Wild-caught
Group Unterstrass
Observations
  • Collected from grass, field (terrestrial)
  • Autumn
  • 15°C
  • Elevation – 571
Putative identification Arthropoda Hexapoda Insecta Orthoptera

Methods
Extraction kit Wizard SV (Promega)
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence Download FASTA    Download AB1
GTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTGCCATCAGGTAATGCTGAGCACTTTAAGGAAACTCCCAGTGATAAGCTGGAGGAAGATGGGGATGATGTCAAGTCATCATGGCCTTTATGAAGTGGGCTACACACGTGCTACAATGGTGTCTACAATGGGCTGCAAGGTGCGCAAGCCTAAGCTAATCCCTAAAAGACATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTTCC
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
ATGAGCTGGATAGTAGGTACATCAATAAGAATAATTATTCGTGCAGAACTTGGACAACCAGGATCCATAATTGGAGATGATCAAATCTATAATGTTATTATTACAGCACATGCATTTGTAATAATTTTCTTCATAGTAATACCTATTATAATTGGAGGATTCGGTAATTGACTGGTTCCACTAATAATCGGAGCACCAGATATAGCTTTTCCACGAATAAATAACATAAGTTTTTGACTTTTACCACCATCATTAACTCTTTTAATTGCATCATCAATAGTAGATAATGGTGCAGGAACAGGATGAACAGTTTATCCCCCTCTCGCAGGAGCAATCGCACATGGAGGAGGATCAGTAGACCTGGCTATTTTTTCATTACATTTAGCAGGTGTTTCATCAATTTTAGGAGCAGTTAATTTCATTACAACTGCAATCAATATACGGTCGGAAAGAATAACACTAGATCAAACACCACTATTTGTCTGATCAGTAGCAATTACAGCACTCCTATTACTATTATCATTACCGGTACTAGCAGGAGCAATTACTATATTATTAACTGATCGAAATTTAAATACTTCGTTCTTTGACCCTGCGGGTGGTGGAGATCCAATCCTTTATCAACATTTATTTTGATTTTTTGGTCACCCTGGAAGTTTAATC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Orthoptera was found to be postive for Wolbachia.
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