Silver Y moth (017)

October 20, 2020

Sample information
Picture
Location No map location data available.
Collection date 09/28/2020
Captive / Cultivated? Wild-caught
Group Unterstrass
Observations
Putative identification Arthropoda Hexapoda Insecta Lepidoptera Noctuidae Autographa Autographa gamma

Methods
Extraction kit Wizard SV (Promega)
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence Download FASTA    Download AB1
TGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTGCTATCAGGTAATGCTGAGTACTTTAAGGAAACTGCCAGTGATAAGCTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCTTTATGGAGTGGGCTACACACGTGCTACAATGGTGTCTACAATGGGCTGCAAGGTGCGCAAGCCTAAGCTAATCCCTAAAAGACATCTCAGTTCGGATTGTACTCTGCAACT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
GGTATTTGAGCTGGAATAGTTGGTACATCTTTAAGATTACTAATTCGAGCAGAATTAGGAACCCCTGGATCTTTAATTGGTGATGATCAAATTTATAATACTATTGTTACAGCTCATGCATTTATTATAATTTTTTTTATAGTTATGCCTATTATAATTGGAGGATTTGGTAATTGACTCGTTCCTCTAATATTAGGAGCTCCTGATATAGCTTTCCCTCGTATAAATAACATAAGTTTTTGACTTTTACCCCCATCTTTAACTCTTTTAATTTCTAGAAGAATTGTAGAAAATGGAGCTGGTACTGGATGAACAGTTTATCCCCCACTTTCATCTAATATCGCCCATGGTGGAAGATCTGTTGATTTAGCTATTTTTTCTTTACATTTAGCTGGAATTTCATCAATTTTAGGAGCAATTAATTTTATTACAACAATTATTAATATACGATTAAATAGTTTATCTTTTGATCAAATACCTTTATTTATCTGAGCTGTTGGAATTACAGCTTTCCTTTTATTACTTTCTTTACCTGTTTTAGCAGGGGCAATTACTATACTTTTAACAGATCGTAATTTAAATACTTCTTTTTTTGATCCTGCTGGAGGAGGAGACCCAATCTTATACCAACATTTATTTTGATTTTTTGGTCACCTGGAGAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Autographa gamma was found to be postive for Wolbachia.
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