Portland State University – Phaneroptera nana

October 31, 2023

Sample information
Picture
Photos by: Jonah R.
Location
Collection date 10/07/2023
Captive / Cultivated? Wild-caught
Group Portland State University
Observations

Found under a leaf in a grassy, forested suburban area
Putative identification Arthropoda Insecta Orthoptera Tettigoniidae Phaneroptera Phaneroptera nana

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Lane 14 is positive for COI, Lane 15 is a Wolbachia positive control, Lane 16 is blank for Wolbachia in the insect.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Phaneroptera nana was found to be negative for Wolbachia.
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