Portland State University – Diptera

October 31, 2023

Sample information
Picture
Photos by: Connor R.
Location
Collection date 10/09/2023
Captive / Cultivated? Wild-caught
Group Portland State University
Observations

Found in an urban park
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Lane 11 is positive for COI, Lane 12 is a Wolbachia positive control, Lane 13 is positive for Wolbachia in the insect.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be postive for Wolbachia.
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