Portland State University – Dermaptera

November 1, 2023

Sample information
Picture
Location No map location data available.
Collection date
Captive / Cultivated? Wild-caught
Group Portland State University
Observations
Putative identification Arthropoda Hexapoda Insecta Dermaptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Lane 14 is positive for COI, Lane 15 has a band for a Wolbachia positive control, and Lane 16 is negative for Wolbachia in the insect.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Dermaptera was found to be negative for Wolbachia.
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