Eastern Carpenter bee

November 2, 2023

Sample information
Picture
Photos by: Mert U.
Location
Collection date 09/15/2023
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Apidae Xylocopa

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All of our controls worked as expected, and the results of my sample did not match the positive control for Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
AATTGGTACAGCAATAAGATTTATTATTCGGATAGAATTAAGAATTCCTGGAAATTGAATTAATAATGATCAAATTTATAATTCATTAGTAACAGCTCATGCTTTTTTAATAATTTTTTTTATAGTTATACCTTTTATAATTGGTGGTTTTGGAAATTGATTAATTCCTTTAATATTAGGATTACCTGATATAGCATTTCCTCGAATAAATAATATTAGTTTTTGATTATTACCACCATCATTAATTTTATTATTAATTAGAAATTTATTTTATCCAAGACCAGGAACTGGTTGAACTATTTATCCTCCTTTATCATCATCTTTATATCATTCTTCACCATCAGTTGATTTAATAATTTTTTCATTACATATTTCAGGAATTTCATCAATTATAGGAGCAATAAATTTTATAGTAACAATTATATTAATAAAAAATATTTCAATAAATTATGATAAAATCAATTTATTTTCATGATCTGTATTTATTACAGCAATTTTATTATTATTATCATTACCAGTATTAGCTGGAGCTATTACTATATTATTATTTGATCGAAATTTTAATACTTCATTTTTTGATCCTATAGGAGGTGGTGATCCAATTTTATTTCAACATTTATTTTGA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Xylocopa was found to be negative for Wolbachia.
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