Green Stink Bug

November 2, 2023

Sample information
Picture
Photos by: Aryaman L.
Location
Collection date 09/17/2023
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations

I found this stink bug on some leaves under a tree at approximately 4 pm. The weather was about 75 degrees at the time of collection. I collected the bug in a box and cut out holes, so that it could breathe. It remained in this box for one day (before dissection).
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All of our controls showed up as we expected. Moreover, my sample did not match the positive control.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
GGNNCAGCAATAAGATNNNNNNTNCNNNNTGNNTTNNGACAACCAGGNNNATTCATTGGAGATGANCAAATNNNNNNTGTNNNNNNNACCGNNNNNNCNTTTGTAANAATTTNNNNNNNNNNNNNNNNNNNNNNNNTTGGAGGNNNNNGAAACNGATNNNNNNNNTTAATNNNNGGAGCACCTGATATAGCATTTCCGCGAATAAATAATATAAGATTTTGATTATTACCACCATCATTAACCTTATTAATAGTAAGAAGATTAGCAGAATCAGGAGCCGGGACTGGATGAACAGTATATCCCCCCTTATCAAGTAATTTATCACATGGAGGAGCATCAGTTGACTTAGCAATTTTTTCCCTACATTTAGCAGGAGTAACATCAATTTTAGGAGCAGTAAATTTTATTTCAACAATTATTANTATACGACCAATAGGAATAACCCCTGAACGAATTCCACTATTCGTTTGATCTGTTGGTATTACTGCATTATTATTATTATTATCACTCCCAGTACTAGCAGGGGCTATTACTATANNATTAACTGATCGAAATTTCAATACATCATTCTTTGACCCATCAGGAGGAGGAGATCCGATTCNTTNTCAACACTNNTTTTTGAT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hemiptera was found to be negative for Wolbachia.
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