BroACC_F23RSW Lepidoptera

November 6, 2023

Sample information
Picture
Photos by: Rana'ya W.
Location
Collection date 08/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Occurred on August 21st, 2023 at 1:42 PM
    • North pond of GSU Armstrong Campus
  • Greenhouse garden bed
  • Capture the insects with a beet stick, canvas, tweezers, and a plastic jar
  • Steps: 
    • Place the canvas under a bush and hit it with the beat stick
    • Collected the insect using a tweezer then placed it in jar
    • Place in the freezer for 10-15 minutes 
    • Store insects in a labeled glass vial with 95% ethanol
Putative identification Arthropoda Hexapoda Insecta Lepidoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

The controls did work I know if my partner and  I took our time and did things slowly results may have differed. there was no + shown but it was a negative control so that made me feel more confident that I can do better next time but all controls were presented besides +.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lepidoptera was found to be negative for Wolbachia.
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