Sample information
Picture	Photos by: Jordyn Owens
Location
Collection date	11/01/2023
Captive / Cultivated?	Wild-caught
Group	John Overton High School
Observations
Putative identification	Arthropoda Chelicerata Arachnida Opiliones
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Whole arthropod
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	Medium
Explanation of confidence level	My confidence level is in the middle because my line on the gel was faded a little.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Opiliones was found to be postive for Wolbachia.