Millipede

April 4, 2025

Sample information
Picture
Photos by: Nicole D
Location
Collection date 11/12/2024
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

Brown, about 4cm long. Found under a rolled over log, curled into a circle when touched & caught.
Putative identification Arthropoda Myriapoda Diplopoda Julida Julidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location posterior to head
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

No results were found on the gel as the gel showed no clear bands. Because of this, none of our samples were sent in for sequencing. This is why we cannot be sure if the millipede had Wolbachia.

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

I can have high confidence that the sample is unknown for Wolbachia because we did not get any results from our DNA extraction. There were almost no bars when our electrophoresis gel was run.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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