Polydesmida – Millipede

November 7, 2023

Sample information
Picture
Photos by: Marcos D. S.
Location
Collection date 11/01/2023
Captive / Cultivated? Wild-caught
Group John Overton High School
Observations

The millipede was found inside of a log. It is likely a detritivore.
Putative identification Arthropoda Myriapoda Diplopoda Polydesmida

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Rear half
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

Our experiment failed, most likely in the DNA extraction step because we did not crush the arthropods enough. Both the Arthropod positive and the DNA positive results were successful, and they gave the expected results. The PCR was successful as well as the Gel electrophoresis, so the DNA extraction was the step that failed.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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