MAC.ACI.F23-#13 Hemiptera

November 10, 2023

Sample information
Picture
Photos by: Ileana F.
Location
Collection date 08/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Time Collected: 1:20 pm
  • Method: Sweep Net
  • Location: In grassy field with tall grass near greenhouse on Armstrong Campus
  • Environment/Habitat: Tall grass in field
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I’m confident that my insect was not infected with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be negative for Wolbachia.
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