LOGACFF23-LB

November 9, 2023

Sample information
Picture
Photos by: Leilianna B.
Location
Collection date 08/22/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Was caught with a sweep net found outside within small plants, the weather was humid and hot, it was 94 degrees outside, it was sunny, caught at 13:20.
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There is not even a hint in the gel electrophoresis that my insect was infected with the Wolbachia 16S C01 gene. It was almost identical to the negative control. I know that I can trust my results because of what I have previously said and there is a low percentage for Hemiptera to be infected with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be negative for Wolbachia.
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