LOGACEF23-HG Hemiptera

November 13, 2023

Sample information
Picture
Photos by: Heather G
Location No map location data available.
Collection date 08/22/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collection method: sweeping net

Collection habitat: grass

Weather conditions: 84 degrees, partly cloudy, slight breeze
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Based on my articles, local, and class prevalence, as well as the gel electrophoresis results, I am very confident that my controls worked properly and that I do not have a false positive.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be postive for Wolbachia.
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