Cricket – CG

November 14, 2023

Sample information
Picture
Photos by: Charlie G
Location
Collection date 09/13/2023
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations

Green, large legs and antennas, jumped a lot while I was trying to catch it.
Putative identification Arthropoda Hexapoda Insecta Orthoptera Tettigoniidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls worked so I am confident that my sample had no Wolbachia
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
TTCACTAAGATTATTAATTCGGGCTGAGCTAGGACAACCAGGCTATCTTATTGGAGACGACCAAATTTAT AACGTTATTGTAACAGCACATGCCTTTGTTATAATTTTTTTTATAGTTATACCAATTATAATCGGAGGAT TCGGAAATTGATTAGTTCCTTTAATACTGGGGGCCCCANACATAGCTTTCCCTCGAATAAACAATATGAN TTTTTGACTTTTGCCCCCATCTTTGACCTTGCTTCTAGCCANAAGCCTTGTCGAAAACGGGGCAGGCACT GGTTGAACAGTTTACCCTCCACTTTCTGCTAATATTGCCCATGCAGGGGCCTCAGTCNATTTAGCAATCT TCTCATTACATTTAGCAGGTATCTCCTCTATTTTAGGAGCTGTAAATTTTATTACTACAACAATTAATAT ACGAGCTCCTGGAATATCCCTGGATCAAACTCCCTTATTTGTATGGGCCGTANCTATTACANCACTACTT TTATTATTATCTTTACCAGTATTAGCTGGAGCTATTACTATATTATTAACTGATCGAAATTTAAATACAT CCTTCTTTGATCCTGCCGGGGGTGGAGACCCGATTCTTTACCAACACTTGTTTTGATTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Tettigoniidae was found to be negative for Wolbachia.
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