Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/13/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Albans School |
| Observations | Green, large legs and antennas, jumped a lot while I was trying to catch it. |
| Putative identification | Arthropoda Hexapoda Insecta Orthoptera Tettigoniidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The controls worked so I am confident that my sample had no Wolbachia |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
TTCACTAAGATTATTAATTCGGGCTGAGCTAGGACAACCAGGCTATCTTATTGGAGACGACCAAATTTAT AACGTTATTGTAACAGCACATGCCTTTGTTATAATTTTTTTTATAGTTATACCAATTATAATCGGAGGAT TCGGAAATTGATTAGTTCCTTTAATACTGGGGGCCCCANACATAGCTTTCCCTCGAATAAACAATATGAN TTTTTGACTTTTGCCCCCATCTTTGACCTTGCTTCTAGCCANAAGCCTTGTCGAAAACGGGGCAGGCACT GGTTGAACAGTTTACCCTCCACTTTCTGCTAATATTGCCCATGCAGGGGCCTCAGTCNATTTAGCAATCT TCTCATTACATTTAGCAGGTATCTCCTCTATTTTAGGAGCTGTAAATTTTATTACTACAACAATTAATAT ACGAGCTCCTGGAATATCCCTGGATCAAACTCCCTTATTTGTATGGGCCGTANCTATTACANCACTACTT TTATTATTATCTTTACCAGTATTAGCTGGAGCTATTACTATATTATTAACTGATCGAAATTTAAATACAT CCTTCTTTGATCCTGCCGGGGGTGGAGACCCGATTCTTTACCAACACTTGTTTTGATTTTT
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Tettigoniidae was found to be negative for Wolbachia. |
Pterostichus permundus
Melanoplus Spretus
Centipede- Lithobius forficatus
Fly- Pollen pediculata
Cricket- Acheta domesticus