Fly–Ava Matheson

November 19, 2023

Sample information
Picture
Photos by: Ava M.
Location
Collection date 09/18/2023
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations
Putative identification Arthropoda Insecta Diptera Calliphoridae Lucilia Lucilia mexicana

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence Download FASTA    Download AB1
TTGnnnGGCTGTCGTCnGCTCGTGTCGTGAGAnGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
TTGnnnnGGTnnAAnCGGAACTTCTTTAAGAATTCTAATTCGAGCTGAATTAGGGCACCCCGGAGCACTAATTGGTGACGATCAAATTTATAATGTAATTGTAACAGCTCATGCTTTTATTATAATTTTCTTTATAGTAATACCAATTATAATCGGAGGGTTTGGAAATTGATTAGTTCCTTTAATATTAGGAGCCCCAGATATGGCATTCCCTCGAATAAATAATATAAGTTTTTGACTCCTCCCTCCTGCATTAACTTTATTATTAGTAAGTAGTATAGTAGAAAACGGAGCTGGAACAGGATGAACAGTTTACCCCCCCTTATCTTCTAATATTGCTCATGGAGGAGCTTCTGTAGATTTAGCTATTTTTTCCCTACATTTAGCCGGGATTTCTTCAATTTTAGGAGCAGTAAATTTTATTACTACAGTTATTAATATACGATCAACAGGAATTACTTTTGACCGAATGCCATTATTTGTTTGATCCGTAGTAATTACAGCTTTATTACTTTTACTA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Lucilia mexicana was found to be postive for Wolbachia.
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