neoscona domiciliorum (spider)

November 20, 2023

Sample information
Picture
Photos by: Sarah S.
Location
Collection date 09/09/2022
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations
Putative identification Arthropoda Chelicerata Arachnida Araneae Araneidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Controls worked as expected.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
NNNNNGGAGCNGGGCTGCTATATTAGGAACAGCAATAAGAGTATTAATTCGAATTGAGTTGGGTCAGCCGGGAAGGTTTATAGGTGATGATCAATTATATAATGTAATTGTAACTGCTCATGCATTTGTAATAATTTTTTTTATAGTAATGCCAATTTTAATTGGGGGGTTTGGTAATTGATTAGTTCCTTTAATATTAGGTGCTCCTGATATAGCTTTTCCCCGAATAAATAATTTAAGATTTTGATTATTACCCCCTTCATTATTATTATTAATTATTTCATCATTAGTTGAAATAGGAGTAGGAGCAGGATGAACTGTATATCCTCCTTTGGCTGGATTAGAAGGACATGCAGGGAGATCAGTAGATTTTGCTATTTTTTCTTTACATTTAGCTGGAGCTTCTTCAATTATAGGTGCAATTAATTTTATTTCTACTATTATTAATATACGTTTTTATGGAATAACTATAGAAAAAGTTCCTTTATTTGTATGGTCTGTAATAATTACAGCTGTATTATTATTATTATCTTTACCTGTATTAGCCGGAGCTATTACAATATTATTAACAGATCGAAATTTTAATACTTCTTTTTTTGACCCGTCTGGGGGTGGAGATCCTATTTTATTTCAACATTTGTTTTGATTTTTNNNNN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Araneidae was found to be negative for Wolbachia.
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