Pisidium rotundatum

November 17, 2023

Sample information
Picture
Photos by: Saanvi V
Location
Collection date 11/13/2023
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

Arthropod was found under leaves inside of a small, shallow, slow-moving river in a tree-shaded area.
Putative identification Mollusca Bivalvia Sphaeriida Sphaeriidae Pisidium

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

We did not see significant results after running gel electrophoresis; therefore, we do not know if the arthropod was wolbachia positive or not.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
CGGTTTGATGGGAACTGCTTTTAGAGTTATTGTTCGTATAGAATTAGCTTTACCTGGTTCATTGTTAAAAGATGCTCAGCTCTATAATGTTTTGGTTACTGCTCATGCTTTAGTAATGATTTTTTTTTTAGTTATACCAATAATAATGGGTGGATTTGGTAATTGATTGGTTCCGCTTATATTAGTGGTTCCTGATATGGCTTTTCCTCGTTTAAATA ATTTAAGATTTTGGTTATTACCTGTTGCATTATTATTGTTATTGGGTTCTGCATATGTGGAAAGGGGTGCTGGAACAGGATGAACTATTTATCCACCTTTATCTGGAAATGTTGCTCATGCTGGTCCTTCTATAGAATTTGCGATTTTATCTTTGCACTTAGGTGGTGCTTCATCTATTGCTGCATCAATTAATTTTTTAACTACTGTTTTAAACATA CGTTTAGGTGTTATAGAGTTAAAACGAGTGAGATTGTTTGTATTTTCTATTGCTATTACTGCTTTTCTATTAATTGTTGCAATACCTGTATTAGCTGGTGGTTTAACAATGCTTTTAACTGATCGAAACTTTAATACTACTTTTTTTGACCCAATAGGAAACGGGGATCCAGTTCTGTTTATACATATTTTTTGATTTTTTGGTCACCCTGGAAGTTTAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary
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