|Captive / Cultivated?||Wild-caught|
|Group||Edmund Burke School|
This cricket is brown on top with a light beige underbelly. Long antennae. Little to differentiate torso from abdomen.
|Putative identification||Arthropoda Hexapoda Insecta|
|Extraction kit||DNeasy (Qiagen)|
|DNA extraction location||Partial abdomen|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||MiniOne|
It was hard to divide up the abdomen as it all kind of turned to goop, so we scooped up what we could and added it to the vial.
We didn’t make any mistakes during the arthropod electrophoresis, but something went wrong for our wolbachia electrophoresis the first time we did it. Luckily, we repeated the experiment and all looks to have gone well.
|Explanation of confidence level||
We did make a mistake the first time we did our wolbachia electrophoresis, but we paid extra attention the next time, and everything looked to have gone perfectly well.
|Wolbachia 16S sequence||
|Arthropod COI sequence|| Download FASTA
AGTCGGCACATCACTAAGTATTTTAATTCGAACGGAATTAGGACAACCAGGGTATCTTATTGGAGATGATCAAACATATA ATGTAATTGTTACTGCACACGCCTTTATTATAATTTTCTTCATAGTAATACCAATTATGATTGGAGGATTCGGAAATTGA TTAGTTCCCTTAATATTAGGAGCACCCGATATAGCTTTCCCCCGAATAAATAATATAAGTTTTTGATTATTACCCCCTTC ATTAACTCTCCTATTAACCAGAAGAATAGTTGAAAATGGAGCAGGTACAGGATGAACAGTTTATCCACCCTTGTCAACAG GAATTGCCCATGCAGGAGCATCAGTTGATTTAGCAATTTTCTCATTACATCTCGCCGGAATCTCCTCCATTTTAGGTGCT GTAAATTTTATTACTACCATAATTAATATACGAGCTCCTAGAATGTCTTTAGATCAAACACCCCTATTTGTGTGAGCAGT AGGAATTACAGCACTATTATTATTATTATCATTACCTGTACTAGCAGGAGCTATTACTATACTATTAACTGATCGAAACT TAAATACCTCATTTTTTGATCCCGCAGGABLAST at The Wolbachia Project BLAST at NCBI
|Summary||The Insecta was found to be negative for Wolbachia.|