Millipede

April 26, 2024

Sample information
Picture
Photos by: Samvidha M.
Location
Collection date 11/13/2023
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

brown, long, thin, many legs, many small segments
Putative identification Arthropoda Myriapoda Diplopoda Julida

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Other
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain
Gel images
Protocol notes

DNA extraction location: 3rd segment of organism

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

When we ran the gels, the controls created the correct bands, but there were some smudged lines in the negative control well. Also, we might not have isolated the right segment of the millipede.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Julida was found to be negative for Wolbachia.
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