Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/13/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | Found unmoving and curled into a circle. Under several leaves and next to a rotting log. Black and brown segmented body. |
| Putative identification | Arthropoda Myriapoda Diplopoda Julida Julidae Cylindroiulus Cylindroiulus caeruleocinctus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | It is possible that I did not accurately extract the reproductive organs of the millipede. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Cylindroiulus caeruleocinctus was found to be negative for Wolbachia. |
Anyphaena accentuata NP1
Cylindroiulus caeruleocinctus NP2
Lithobius forficatus SM1
Oniscus asellus GC1
Oniscus Asellus (LS1)