Cylindroiulus caeruleocinctus

April 26, 2024

Sample information
Picture
Photos by: Allison Ray
Location
Collection date 11/13/2023
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

Found unmoving and curled into a circle. Under several leaves and next to a rotting log. Black and brown segmented body.
Putative identification Arthropoda Myriapoda Diplopoda Julida Julidae Cylindroiulus Cylindroiulus caeruleocinctus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

It is possible that I did not accurately extract the reproductive organs of the millipede.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Cylindroiulus caeruleocinctus was found to be negative for Wolbachia.
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