Sample information
Picture	Photos by: MAximilian S., FElix L., Floyd S.
Location
Collection date	11/09/2023
Captive / Cultivated?	Wild-caught
Group	Gymnasium Immensee
Observations	At the facade of the house from the Gymnasium Immensee near the forest
Putative identification	Arthropoda Hexapoda Insecta Diptera Culicidae Culex Culex pipiens
Methods
Extraction kit	BABEC
DNA extraction location	Whole arthropod
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	MiniOne
Buffer	TAE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level	First time doing this :)
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Culex pipiens was found to be negative for Wolbachia.