Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/09/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Gymnasium Immensee |
| Observations |
|
| Putative identification | Arthropoda Hexapoda Insecta Diptera Culicidae |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | We had a very good result after the gel electrophoresis. We also worked under sterile circumstances and had 2 control DNA’s. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Culicidae was found to be negative for Wolbachia. |
We caught the mosquito in the forest
Pterostichus permundus
Melanoplus Spretus
Centipede- Lithobius forficatus
Fly- Pollen pediculata
Cricket- Acheta domesticus