1-ASH

March 5, 2024

Sample information
Picture
Photos by: Alex S.
Location
Collection date 09/13/2023
Captive / Cultivated? Wild-caught
Group Woburn Memorial High School
Observations
  • Legs have a gradient from light tan at the base to black at the tips
  • Overall light tan in color
  • Darker diamond-like pattern visible on abdomen
  • Cephalothorax and Abdomen are roughly 1.2 mm in length
Putative identification Arthropoda Arachnida Opiliones Phalangiidae Phalangium Phalangium opilio

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

Phalangium opilio DNA was not present in the gel electrophoresis results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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