1-SK

March 5, 2024

Sample information
Picture
Photos by: Sylvia K.
Location
Collection date 09/12/2023
Captive / Cultivated? Wild-caught
Group Woburn Memorial High School
Observations

This small, eight-legged arthropod has a large abdomen and a large cephalothorax. It is about a centimeter in length.
Putative identification Arthropoda Chelicerata Arachnida Araneae Theridiidae Parasteatoda Parasteatoda tepidariorum

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The results of all four of the controls were as expected: there was no contamination in the control with only water, the CO1 gene was visible in the other three controls, and the 16S rRNA gene was visible in the positive Wolbachia controls (refer to wells four to seven in image one and wells six to nine in image two). These results ensured there was no DNA contamination, and DNA extraction and PCR worked. Specimen 1-SK had the CO1 gene but not the 16S rRNA Wolbachia gene (refer to well two in image one and wells two and three in image two). Since the controls worked correctly, it can be concluded that 1-SK was not infected with Wolbachia.
Wolbachia 16S sequence
N/A
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
N/A
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Parasteatoda tepidariorum was found to be negative for Wolbachia.
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