Pollenia pediculata,

April 25, 2024

Sample information
Picture
Photos by: Jaime R.
Location
Collection date 03/27/2024
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations

J3 through J6 tested for Wolbachia gene 16s, J3-Wasp, J4-large cricket, J5-Fly, J6-Small cricket, J7 through J10 tested for Arthropod gene Co1, J7-wasp, J8-Large cricket, J9-Fly, J10-Small cricket, arrows indicate positive gene, J5-tested pos. for 16s, J7+J9 tested pos. For Co1
Putative identification Arthropoda Insecta Diptera Polleniidae Pollenia Pollenia pediculata

Methods
Extraction kit Monarch DNA extraction (NEB)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer LB
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
jaime_samp5_WO_Wolbachia_pipientis_strain GTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCC
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
jaime_samp5_CO1_7C_Pollenia_pediculata_voucher TCTTTAAGAATATTAGTTCGAGCAGAATTAGGACATCCTGGAGCATTAATCGGTGATGATCAAATTTATAATGTAATTGTAACAGCCCATGCCTTTGTTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGACTTGTTCCTTTAATATTAGGAGCACCTGATATAGCATTCCCTCGAATGAATAATATAAGTTTTTGACTCTTACCTCCTGCACTTACATTATTATTGGTGAGCAGTATAGTGGAAAACGGAGCTGGGACAGGATGAACTGTTTACCCACCTCTATCTTCTAATATTGCTCATGGAGGGGCTTCTGTTGATTTAGCTATTTTTTCTCTTCATTTAGCAGGAATTTCTTCAATTTTAGGAGCAGTTAATTTTATTACAACTGTAATTAATATACGATCTACAGGTATTACATTTGACCGAATACCTTTATTTGTTTGATCTGTAGTAATTACAGCATTATTACTTCTTTTATCCTTACCTGTATTAGCAGGAGCTATCACTATATTATTAACAGATCGAAATTTAAATACTTCCTTTTTTGATCCTGCAGGAGGAGGAGACCCAATTTTATATCAACA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Pollenia pediculata was found to be postive for Wolbachia.
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