Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/05/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | Collected by Fall 2023 |
| Putative identification | Arthropoda Hexapoda Insecta Lepidoptera Nymphalidae Phyciodes Phyciodes tharos |
Methods |
|
| Extraction kit | Qiagen Blood and Tissue Kit - With Modifications - P2 for ATL and N3 for AL |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | I think I did all of the procedure right but there is still room for error. |
| Wolbachia 16S sequence |
None.
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence |
GAAGTGTTAATATTTCGATCAGTTAAAAGTATTGTAATAGCACCAGCTAATACTGGTAATGAAAGTAATAATAATAAGGCTGTAATACCGACAGCTCAAACGAATAAAGGTATTTGATCAAAGGATATATTATTAACACGTATATTAATAATTGTAGTAATAAAATTAATAGCTCCTAAAATTGATGAAATTCCTGCTAAATGAAGGGAAAAAATTGCTAAATCTACTGAAGCTCCTCTATGGGCAATATTAGATGAAAGGGGTGGGTAAACTGTTCATCCTGTTCCTGCTCCATTTTCTACGATTCTACTAGAAATTAATAAAATTAATGACGGGGGTAGTAATCAAAAACTTATATTATTTATTCGGGGGAAAGCTATATCAGGAGCTCCTAATATTAAAGGTACTAATCAATTTCCAAATCCTCCAATTATAATAGGTATAACTATAAAAAAAATTATAATAAAAGCATGAGCTGTAACAATAGTATTATAAATTTGATCATCTCATTAAAGANCCGGGANTTCCTAATTCAGTTCNAATTAANANCANANAATCCTCCTGCNATAAATA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Phyciodes tharos was found to be negative for Wolbachia. |
Water Boatman