Phyciodes tharos

April 25, 2024

Sample information
Picture
Photos by: Ruzaina S.
Location
Collection date 09/05/2023
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2023
Putative identification Arthropoda Hexapoda Insecta Lepidoptera Nymphalidae Phyciodes Phyciodes tharos

Methods
Extraction kit Qiagen Blood and Tissue Kit - With Modifications - P2 for ATL and N3 for AL
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I think I did all of the procedure right but there is still room for error.
Wolbachia 16S sequence
None.
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
GAAGTGTTAATATTTCGATCAGTTAAAAGTATTGTAATAGCACCAGCTAATACTGGTAATGAAAGTAATAATAATAAGGCTGTAATACCGACAGCTCAAACGAATAAAGGTATTTGATCAAAGGATATATTATTAACACGTATATTAATAATTGTAGTAATAAAATTAATAGCTCCTAAAATTGATGAAATTCCTGCTAAATGAAGGGAAAAAATTGCTAAATCTACTGAAGCTCCTCTATGGGCAATATTAGATGAAAGGGGTGGGTAAACTGTTCATCCTGTTCCTGCTCCATTTTCTACGATTCTACTAGAAATTAATAAAATTAATGACGGGGGTAGTAATCAAAAACTTATATTATTTATTCGGGGGAAAGCTATATCAGGAGCTCCTAATATTAAAGGTACTAATCAATTTCCAAATCCTCCAATTATAATAGGTATAACTATAAAAAAAATTATAATAAAAGCATGAGCTGTAACAATAGTATTATAAATTTGATCATCTCATTAAAGANCCGGGANTTCCTAATTCAGTTCNAATTAANANCANANAATCCTCCTGCNATAAATA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Phyciodes tharos was found to be negative for Wolbachia.
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