GRP 19 (B19) – Dilophus

May 24, 2024

Sample information
Picture
Photos by: Cael C., Austin K., Ryan B. & Luis L.
Location
Collection date 05/14/2024
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

Arthropod caught on tree by lunch benches at CAMS
Putative identification Arthropoda Hexapoda Insecta Diptera Bibionidae Dilophus

Methods
Extraction kit tweezers
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain UView
Gel images
Protocol notes

Both gels ran for just over an hour, before being examined for bands

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

The Wolbachia gel produced almost no evidence. The bands were either too faint to read or the DNA ran off the gel, presumably because of a PCR error causing the Wolbachia samples to not be amplified enough.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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