Group 5 CAMS 2024 – Wolbachia in the 90250

Sample information
Picture	Photos by: Diego DL
Location
Collection date	05/22/2024
Captive / Cultivated?	Wild-caught
Group	California Academy of Mathematics and Science
Observations	Came toward the smell of banana peel and day-old eaten apple. Very large fly, buzzed very loud, and was seemingly easy to catch with petri dish.
Putative identification	Arthropoda Hexapoda Insecta Diptera Drosophilidae
Methods
Extraction kit	none
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	Fast Blast
Gel images
Protocol notes	100 volt, 90 minutes.
Results
Wolbachia presence	Yes
Confidence level	High
Explanation of confidence level	Lane 1 (whichever side of the gel has an instant dye front), contains control, and the second lane contains this arthropod. The fourth lane also contains the positive Wolbachia, where they all have a band at the same distance.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Drosophilidae was found to be postive for Wolbachia.

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