Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/22/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | California Academy of Mathematics and Science |
| Observations | Came toward the smell of banana peel and day-old eaten apple. Very large fly, buzzed very loud, and was seemingly easy to catch with petri dish. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Drosophilidae |
Methods |
|
| Extraction kit | none |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | 100 volt, 90 minutes. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | Lane 1 (whichever side of the gel has an instant dye front), contains control, and the second lane contains this arthropod. The fourth lane also contains the positive Wolbachia, where they all have a band at the same distance. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Drosophilidae was found to be postive for Wolbachia. |
Water Boatman