GRP15 Oleander Aphid

Sample information
Picture	Photos by: Maegan A., Faith D., Kristine F., Christine M.
Location
Collection date	05/15/2024
Captive / Cultivated?	Wild-caught
Group	California Academy of Mathematics and Science
Observations	Arthropod was found in the school garden in clusters on the stems of milkweed in front of classroom.
Putative identification	Arthropoda Hexapoda Insecta Hemiptera Aphididae
Methods
Extraction kit	tweezers
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	Fast Blast
Gel images
Protocol notes	Both gels ran for 35 minutes at 135 volts and 70 amps. Results were viewed w/ a white light box. Wells starting new ruler (Gel 1: Arthropod) Lane 1: DNA Ladder Lane 2: Aphids DNA Lane 3: Ants DINA Lane 4: (+) Arthropod Control Lane 5: (-) Arthropod Control Lane 6: DNA Control Lane 7: Water Wells starting new ruler (Gel 2: Wolbachia) Lane 1: DNA Ladder Lane 2: Aphids DNA Lane 3: Ants DINA Lane 4: (+) Wolbachia Control Lane 5: (-) Wolbachia Control Lane 6: DNA Control Lane 7: Water
Results
Wolbachia presence	Unknown
Confidence level	High
Explanation of confidence level	Both gels did not detect visual evidence of bands when running gel electrophoresis. After consideration of the protocol, the error must have occurred in the PCR process for the DNA controls and ladder were not apparent in their designated lanes.
Wolbachia 16S sequence
Arthropod COI sequence
Summary

The Wolbachia Project Database