Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/22/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | California Academy of Mathematics and Science |
| Observations | Small fly, attracted to fruit but was caught by trapping it in a bag with dog feces. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Drosophilidae |
Methods |
|
| Extraction kit | none |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | 100 Volts, 90 minutes |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | DNA extraction failed, as the third lane containing this arthropod did not have bands. We know it’s DNA extraction because Lane 2, containing a different arthropod, had bands and underwent the same PCR, and had a band. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Water Boatman