Group 5 CAMS 2024 – More Wolbachia, Except this time in LB

May 24, 2024

Sample information
Picture
Photos by: Diego DL
Location
Collection date 05/22/2024
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

Small fly, attracted to fruit but was caught by trapping it in a bag with dog feces.
Putative identification Arthropoda Hexapoda Insecta Diptera Drosophilidae

Methods
Extraction kit none
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

100 Volts, 90 minutes

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

DNA extraction failed, as the third lane containing this arthropod did not have bands.  We know it’s DNA extraction because Lane 2, containing a different arthropod, had bands and underwent the same PCR, and had a band.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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