TriangleEye01

Sample information

Picture
Photos by: Sian G.
Location
Collection date 08/20/2024
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations

in garden in a yew hedge

Putative identification Arthropoda Chelicerata Arachnida Araneae

Methods

Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer
DNA stain Midori 1:20'000
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level Low
Explanation of confidence level

our use of the pipets was a bit too quick and imprecise

we might have used too much of the MASTER-Mix

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Araneae was found to be negative for Wolbachia.
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