Tingidae (Netzwanze)

Sample information

Picture
Photos by: Elin H., Riana E.
Location
Collection date 08/21/2024
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations

This arthropod was found in a garden on a leaf of a raspberry bush in the late afternoon.

Putative identification Arthropoda

Methods

Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer
DNA stain Midori 1:20'000
Gel images
Protocol notes

This sample showed the DNA in the second PCR run. More precisely, in our first PCR no results were observable. Given that there was a result in the second time, there must have occured an error in the PCR process (e.g incorrect amount of a solution, wrong order of steps or wrong execution of single steps).

Results

Wolbachia presence Yes
Confidence level High
Explanation of confidence level

First and foremost, this PCR process carried out and controlled by my biology teacher. In our first PCR attempt, there clearly was DNA but it did not approach the positive pole on the gel but stayed at the top. However, in the second PCR process, supervised by our biology teacher, it worked out and the lines clearly visibly near the positive pole.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be postive for Wolbachia.
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