October 22, 2024

Sample information
Picture
Location
Collection date 08/26/2024
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations
Putative identification

Methods
Extraction kit Instagene Matrix
DNA extraction location Other
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer
DNA stain
Gel images
Protocol notes

We only got one Wolbachia band. This could be due to the DNA extraction process. Especially where we tried to take out the ovaries of our arthropodes. This was very difficult and didn’t work out well so we used a big part of the abdomen instead which might’ve lead to problems during the PCR.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

We only had one Arthropode which showed a Wolbachia band but we’re very confident that it’s positive because the band is very strong.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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