Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | |
| Captive / Cultivated? | Wild-caught |
| Group | Kantonsschule Zuercher Unterland |
| Observations | This leafhopper was caught on the ground in a flower bed which is located in the garden. |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | |
| DNA stain | Midori 1:20'000 |
| Gel images |
|
| Protocol notes | This sample showed the DNA in the second PCR run. More precisely, in our first PCR no results were observable. Given that there was a result in the second time, there must have occured an error in the PCR process (e.g incorrect amount of a solution, wrong order of steps or wrong execution of single steps). |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | First and foremost, this PCR process carried out and controlled by my biology teacher. In our first PCR attempt, there clearly was DNA but it did not approach the positive pole on the gel but stayed at the top. However, in the second PCR process, supervised by our biology teacher, it worked out and the lines clearly visibly near the positive pole. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Arthropoda was found to be postive for Wolbachia. |
Water Boatman