Sample information |
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Picture |
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Location | |
Collection date | 08/25/2024 |
Captive / Cultivated? | Wild-caught |
Group | Kantonsschule Zuercher Unterland |
Observations | Captured: At home in the house in Niederhasli |
Putative identification | Arthropoda Hexapoda Insecta Blattodea Ectobiidae Blattella Blattella germanica |
Methods |
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Extraction kit | Instagene Matrix |
DNA extraction location | Abdomen |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | MiniOne |
Buffer | TBE |
DNA stain | Midori 1:20'000 |
Gel images |
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Protocol notes | In both PCR processes meaningful/good results could be observed. We refer to the results in lane 5 (picture 1) and lane 1 (picture 2). |
Results |
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Wolbachia presence | No |
Confidence level | High |
Explanation of confidence level | There is no mark on the gel. The first try of the PCR process, done by us students, failed but the second one, done by our teacher, showed clear results. Sequencing failed —> very poor quality, possible cause: problems during transportation We took the name of the arthropod that we initially thought it was (Blattella germanica). |
Wolbachia 16S sequence | |
Arthropod COI sequence | Download AB1
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Summary | The Blattella germanica was found to be negative for Wolbachia. |