Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/25/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Kantonsschule Zuercher Unterland |
| Observations | Captured: In the garden at home in Oberweningen Condition: Alive when caught and then frozen to death and then preserved in alcohol |
| Putative identification | Arthropoda Hexapoda Insecta Diptera |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | Midori 1:20'000 |
| Gel images |
|
| Protocol notes | In both PCR processes meaningful/good results could be observed. We refer to the results in lane 6 (picture 1) and lane 2 (picture 2). |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | There is no mark on the gel. The first try of the PCR process, done by us students, failed but the second one, done by our teacher, showed clear results. Sequencing did not work, so we took the name of the arthropod that we initially thought it was (Diptera). |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Diptera was found to be negative for Wolbachia. |
Water Boatman