Diptera_Herz_04

October 24, 2024

Sample information
Picture
Photos by: Lea R., Louisa R.
Location
Collection date 08/25/2024
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations

Captured: In the garden at home in Oberweningen

Condition: Alive when caught and then frozen to death and then preserved in alcohol
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain Midori 1:20'000
Gel images
Protocol notes

In both PCR processes meaningful/good results could be observed. We refer to the results in lane 6 (picture 1) and lane 2 (picture 2).

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There is no mark on the gel. The first try of the PCR process, done by us students, failed but the second one, done by our teacher, showed clear results.

Sequencing did not work, so we took the name of the arthropod that we initially thought it was (Diptera).
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
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