Formica cinerea_Kreis_1

October 24, 2024

Sample information
Picture
Photos by: Leandro P
Location
Collection date 08/25/2024
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations

I found it on a gras field in a neighbourhood.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Formica Formica cinerea

Methods
Extraction kit Instagene Matrix
DNA extraction location body without wings
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain Midori 1:20'000
Gel images
Protocol notes

Reasons why it didn‘t work during the first try (Picture 1):

– Foam formation (broken machine)

– to much/few sample pipetted in the gel

– Centrifuged for too little time

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Formica cinerea (Aschgraue Sklavenameise)

https://de.wikipedia.org/wiki/Aschgraue_Sklavenameise

DNA tested
Wolbachia 16S sequence Download FASTA    Download AB1
Arthropod COI sequence Download FASTA    Download AB1
Summary The Formica cinerea was found to be postive for Wolbachia.
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