Notostira erratica Davidstern 02

October 24, 2024

Sample information
Picture
Photos by: Shubham und Dario Gruppe Davidstern
Location
Collection date 08/25/2024
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations

On a little hill behind a tree in the high gras. I cought it with a big net.
Putative identification Arthropoda Hexapoda Insecta Hemiptera Miridae Notostira Notostira erratica

Methods
Extraction kit Instagene Matrix
DNA extraction location body without wings
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain Midori 1:20'000
Gel images
Protocol notes

Runned smothly, first lane in the image  mid faint

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

After the Blast, it showed 89.98% with Notostira erratica but 86.44% with Ceratocapsus sp. After searching the both Names we compared the pictures and it was the Notostira erratica that look similar when not exactly the same as our pictures from the Insect. So we are confident that it is the Notostira erratica.

Picture of a Notostira erratica from Wikipedia as a comparison.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
TCNTTAANANGAATTATTCGTATTGAACTANGAATACCTGNNNNNNTTATTGGAGATGATCAAACATATAATGTTGTAGT AACANCTCACGCATTCATCATAATCTTTTTTATANTAANNNNNNTTANNNNTGGGNNNNNNNNNNNNNNNNNNNNNCCNT TAANAATTNNNNCNNNNNNNNNNNNNNNNCCNCNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNCCNTAT NATTAT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Notostira erratica was found to be negative for Wolbachia.
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