Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/25/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Kantonsschule Zuercher Unterland |
| Observations | On a little hill behind a tree in the high gras. I cought it with a big net. |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera Miridae Notostira Notostira erratica |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | body without wings |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | Midori 1:20'000 |
| Gel images |
|
| Protocol notes | Runned smothly, first lane in the image mid faint |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | After the Blast, it showed 89.98% with Notostira erratica but 86.44% with Ceratocapsus sp. After searching the both Names we compared the pictures and it was the Notostira erratica that look similar when not exactly the same as our pictures from the Insect. So we are confident that it is the Notostira erratica.
Picture of a Notostira erratica from Wikipedia as a comparison. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
TCNTTAANANGAATTATTCGTATTGAACTANGAATACCTGNNNNNNTTATTGGAGATGATCAAACATATAATGTTGTAGT AACANCTCACGCATTCATCATAATCTTTTTTATANTAANNNNNNTTANNNNTGGGNNNNNNNNNNNNNNNNNNNNNCCNT TAANAATTNNNNCNNNNNNNNNNNNNNNNCCNCNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNCCNTAT NATTAT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Notostira erratica was found to be negative for Wolbachia. |
Water Boatman