Sample information |
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Picture |
|
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Location | |
Collection date | 08/25/2024 |
Captive / Cultivated? | Wild-caught |
Group | Kantonsschule Zuercher Unterland |
Observations | On a little hill behind a tree in the high gras. I cought it with a big net. |
Putative identification | Arthropoda Hexapoda Insecta Hemiptera Miridae |
Methods |
|
Extraction kit | Instagene Matrix |
DNA extraction location | body without wings |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | MiniOne |
Buffer | TBE |
DNA stain | Midori 1:20'000 |
Gel images |
|
Protocol notes | |
Results |
|
Wolbachia presence | No |
Confidence level | High |
Explanation of confidence level | Tested from the teacher, analysed every mistake that could have happend, teacher made it after we did it a second time |
Wolbachia 16S sequence | |
Arthropod COI sequence |
|
Summary | The Miridae was found to be negative for Wolbachia. |