Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/25/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Kantonsschule Zuercher Unterland |
| Observations | On a little hill behind a tree in the high gras. I cought it with a big net. |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera Miridae |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | body without wings |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | Midori 1:20'000 |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Tested from the teacher, analysed every mistake that could have happend, teacher made it after we did it a second time |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Miridae was found to be negative for Wolbachia. |
Spider TBD