Lasius (Kreis)

Sample information

Picture
Photos by: Leandro P
Location
Collection date 08/25/2024
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations

I found it on a gras field in a neighbourhood.

Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Lasius Lasius niger

Methods

Extraction kit Instagene Matrix
DNA extraction location body without wings
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain Midori 1:20'000
Gel images
Protocol notes

Reasons why it didn‘t work during the first try:

– Foam formation (broken machine)

– to much/few sample pipetted in the gel

– Centrifuged for too little time

Results

Wolbachia presence Yes
Confidence level High
Explanation of confidence level

2nd try executed by the teacher

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lasius niger was found to be postive for Wolbachia.
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