coenosia atra

October 24, 2024

Sample information
Picture
Photos by: Lea R., Louisa R.
Location
Collection date 08/25/2024
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations

captured: on the wall of the garage at home in Oberweningen

condition: caught alive, then frozen to death and preserved in alcohol
Putative identification Arthropoda Arachnida Araneae

Methods
Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain Midori 1:20'000
Gel images
Protocol notes

In the PCR process no results could be observed. There must have happened a mistake during the DNA extraction. We refer to lane 7 (picture 1).

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

coenosia atra
(eng: tiger fly)
picture: http://www.insektenbox.de/zweifl/coeatrm1.htm

We are quite sure that the result is correct because it says that the anthropoid is a coenosia and we have caught a coenosia. In addition, the DNA sequence is quite long and therefore meaningful, which indicates good reliability.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary
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